Reproduction Abstracts

The hollow fiber vitrificaiton (HFV) method, we developed (Matsunari et al., JRD 58, 2012) has been shown to be very effective in the cryopreservation of highly cryosensitive embryos, such as in vitro matured-fertilized pig morulae. The objective of this study was to verify the effectiveness of the HFV method to the in vitro matured-fertilized bovine embryos in early developmental stage. Crossbred (Holstein×Japanese Black) in vitro matured-ferti...

In our previous study, we demonstrated that pigs expressing neonatally lethal phenotype induced by genetic modification could be rescued by induction of chimerism by using blastocyst complementation. Here, we show that the obtained chimeric pigs can faithfully transmit the genotype, which is the cause of the lethal traits, to progenies.Cloned embryos (male) of pigs showing the phenotype of pancreatogenesis deficiency by expression of the Pdx1-Hes1</e...